anti tumor necrosis factor alpha tnf α antibody  (Bioss)

Journal: Iranian Journal of Pharmaceutical Research : IJPR

Article Title: Probiotics Ameliorate Atrial Fibrillation-Associated Biomarkers and Inflammation in Rats via Modulation of the Gut Microbiota and NLRP3 Inflammasome Pathway

doi: 10.5812/ijpr-168612

Figure Lengend Snippet: Probiotics improved atrial fibrillation (AF) by regulating intestinal flora imbalance and the NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome signaling pathway in rats. The expression of AF biomarkers pentraxin 3 (PTX3), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), chemerin, and Gal-3 was detected by Western blot (A) and real-time quantitative polymerase chain reaction (RT-qPCR) (B). n = 5; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The membranes were blocked with 5% skim milk at room temperature for 1 h and subsequently incubated overnight at 4°C with the following primary antibodies: Anti-NLRP3 antibody (68102-1-lg, Proteintech), Anti-caspase-1 antibody (HA722222, Huane Bio), Anti-IL-1β antibody (26048-1-ap, Proteintech), Anti-pentraxin 3 (PTX3) antibody (DF8762, Affinity), Anti-IL-6 antibody (ab9324, abcam), Anti-tumor necrosis factor-alpha (TNF-α) antibody (bs-0549R, Bioss), Anti-chemerin antibody (833550-4-RR, Proteintech), Anti-Galectin-3 antibody (60207-1-Ig, Proteintech), and Anti-GAPDH antibody (AF7021, Affinity).

Techniques: Probiotics, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Molecular Medicine Reports

Article Title: Polygonatum sibiricum polysaccharide alleviates liver fibrosis through the TGF-β/Smad signaling pathway and reduces collagen

doi: 10.3892/mmr.2025.13599

Figure Lengend Snippet: PSP inhibits hepatic collagen production in liver fibrosis-induced rats. Detection of the liver fibrosis markers (A) HA, (B) LN, (C) PIIINP and (D) Col IV in serum samples was performed by ELISA. (E) Expression levels of Col I and Col III in the liver were detected by immunohistochemistry; scale bar, 20 µm. Semi-quantification of (F) Col I and (G) Col III. ELISA was used to detect the levels of (H) Col I and (I) Col III in liver tissue. (J) Levels of Hyp in the liver tissues were detected by ELISA. Data are presented as the mean ± standard deviation, n=6. ### P<0.001 vs. the control group; *P<0.05, **P<0.01 and ***P<0.001 vs. the model group. BJRG, Biejia ruangan; Col, collagen; H, high; HA, hyaluronic acid; Hyp, hydroxyproline; L, low; LN, laminin; PIIINP, procollagen III N-terminal peptide; PSP, Polygonatum sibiricum polysaccharide.

Article Snippet: Goat serum (cat. no. c-0005), MMP2 (cat. no. bs4605R), MMP9 (cat. no. bsm55544m), Col III (cat no. bs-0549R) and Col I (cat no. bs-7158R) antibodies were obtained from Bioss.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Standard Deviation, Control

Journal: World Journal of Stem Cells

Article Title: Aligned nanofiber scaffolds combined with cyclic stretch facilitate mesenchymal stem cell differentiation for ligament engineering

doi: 10.4252/wjsc.v17.i8.107124

Figure Lengend Snippet: Effects of static and tensile conditions on bone marrow-derived mesenchymal stem cell alignment and tenogenic differentiation on aligned and random nanofiber scaffolds. A: Schematic showing the experimental setup for culturing bone marrow-derived mesenchymal stem cells (BMSCs) on aligned and random nanofiber scaffolds under static and tensile strain conditions; B: Immunofluorescent staining of F-actin (red) and nuclei (4’,6-diamidino-2-phenylindole, blue) illustrating cytoskeletal alignment of BMSCs in response to static and tensile conditions. Under tensile strain, BMSCs on both scaffold types align along the direction of strain (yellow arrows). Scale bars: 50 μm; C: Gene expression levels of tenogenic markers collagen type I alpha 2, collagen type III alpha 1, tenascin C, and tenomodulin in BMSCs on aligned and random scaffolds under static and tensile conditions, showing upregulation of these markers with tensile strain; D: Western blot analysis of tenogenic proteins (collagen I, collagen III, tenascin C, and tenomodulin) in BMSCs cultured on aligned and random scaffolds under static and tensile conditions, with β-actin as the loading control; E: Quantified protein expression levels from western blot, demonstrating scaffold orientation and mechanical strain effects on tenogenic protein expression. a P < 0.05, b P < 0.01. DAPI: 4’,6-diamidino-2-phenylindole; Col1a2: Collagen type I alpha 2; Col3a1: Collagen type III alpha 1.

Article Snippet: Western blotting was performed, and proteins were detected using specific primary antibodies against collagen I (BIOSS, BS-10423R, Beijing, China), collagen III (BIOSS, BS-0549R, Beijing, China), tenascin C (BIOSS, BS-1039R, Beijing, China), and tenomodulin (BIOSS, BS-7525R, Beijing, China), with β-actin (BIOSS, BS-20735R, Beijing, China) as a loading control.

Techniques: Derivative Assay, Staining, Gene Expression, Western Blot, Cell Culture, Control, Expressing

Journal: World Journal of Stem Cells

Article Title: Aligned nanofiber scaffolds combined with cyclic stretch facilitate mesenchymal stem cell differentiation for ligament engineering

doi: 10.4252/wjsc.v17.i8.107124

Figure Lengend Snippet: Effects of Ras homolog gene family (Rho)-associated coiled coil-containing kinase inhibition with Y27632 on bone marrow-derived mesenchymal stem cell tenogenic differentiation and cytoskeletal organization under static and tensile conditions. A: Schematic showing the experimental setup for culturing bone marrow-derived mesenchymal stem cell (BMSCs) on aligned and random nanofiber scaffolds under static and tensile conditions with Y27632 treatment; B: Immunofluorescent staining of F-actin (red) and nuclei (4’,6-diamidino-2-phenylindole, blue) illustrating the cytoskeletal alignment of BMSCs in response to static and tensile conditions with Y27632. Under tensile strain, BMSCs on both scaffold types align along the strain direction (yellow arrows). Scale bars: 50 μm; C: Western blot analysis of tenogenic proteins (collagen I, collagen III, tenascin C, and tenomodulin) in BMSCs on aligned and random scaffolds under static and tensile conditions with Y27632, with β-actin as the loading control; D: Quantification of protein expression levels, showing scaffold orientation and mechanical strain effects on tenogenic protein expression in the presence of Y27632; E: Expression analysis of Ras homolog gene family (Rho)-associated coiled coil-containing kinase, focal adhesion kinase, and runt-related transcription factor 2 at both gene and protein levels, demonstrating the effects of Y27632 on tenogenic signaling pathways in BMSCs under static and tensile conditions on aligned and random scaffolds. a P < 0.05, b P < 0.01. DAPI: 4’,6-diamidino-2-phenylindole; ROCK: Ras homolog gene family (Rho)-associated coiled coil-containing kinase; FAK: Focal adhesion kinase; RUNX2: Runt-related transcription factor 2.

Article Snippet: Western blotting was performed, and proteins were detected using specific primary antibodies against collagen I (BIOSS, BS-10423R, Beijing, China), collagen III (BIOSS, BS-0549R, Beijing, China), tenascin C (BIOSS, BS-1039R, Beijing, China), and tenomodulin (BIOSS, BS-7525R, Beijing, China), with β-actin (BIOSS, BS-20735R, Beijing, China) as a loading control.

Techniques: Inhibition, Derivative Assay, Staining, Western Blot, Control, Expressing, Protein-Protein interactions